Purification and characterization of lipase produced by indigenously isolated bacteria

Muhammad Aqeel Sultan

doi:10.19045/bspab.2020.90268

Abstract

Bacteria including Bacillus species often produce extracellular enzymes to degrade substances such as proteins, lipids, and starch. Glycerol and long-chain fatty acids are hydrolyzed by lipases (triacylglycerol acylhydrolases, EC 3.1.1.3) to generate esters. Bacterial strains were isolated from rotten fruits and vegetables and cultured at 20 to 50 0C with different types of substrates (Olive oil, Mustard oil and Tributryne). Extracellular enzymes produces from bacteria with different physical parameter and also using different substrates. Only two strains, strain 1 and strain 2 show results when provided substrate Olive oil and Mustard oil at pH 7 and at temperature 300C but Tributryne show result at pH 9 and at temperature 400C. Addition of maltose and fructose increased the lipase activity to 28.53U/ mL and 30.17U/mL respectively. The activity enhance to 50.83U/mL by the addition of yeast extract as nitrogen source. Addition of 60% ammonium sulfate results in maximum precipitation with activity 59.46 U/mL. The results concluded that isolated bacterial strains are good producer of lipase enzyme. The purified enzyme could be a good candidate for various industrial applications. Keywords: Ammonium sulfate precipitation; Bacillus species; Lipase activity; Rotten fruits; Tributryne agar Plate http://dx.doi.org/10.19045/bspab.2020.90268

Highlights

Isolation of bacteria from rotten fruits and vegetables High amount of bacteria is present in contaminated sites with organic waste revealed by microbiological analysis of rotten fruits and vegetables
Effect of Temperature on Lipase The results obtained during this research shows that lipase produce by isolated bacterial strains have good activity from 30 0C to 40 0C with varying substrate
The maximum activity was obtained at 30 0C with olive oil and Mustard oil as substrates and 40 0C with trybutryne as a substrate

Summary

Introduction

The earlier cultures can be used to check the better development of biochemistry, many enzyme-mediated processes. Processes like fermentation recovery or around 4000 enzymes were discovered producing many number of enzymes at from which 200 are used in various reasonable cost. The source of most of the improvements in methods of using enzymes manufacturing enzymes are different on request have significantly increasing. Enzymes can catalyze due to many dollars trades of enzymes per annum till diverse revolutions, in market enzymes. 1960, but later enormously developed taken number continues to proliferate.

Objectives

Results

Conclusion