Abstract
Hypoxanthine/guanine phosphoribosyltransferase was purified from bovine snout epidermis, about 600-fold by a combination method of centrifugation, ammonium sulfate fraction, Sephadex G-200 and DEAE cellulose chromatography. Enzymatic properties of the purified enzyme were determined as follows: pH optimum 7.2, temperature optimum 56 degrees C, and 82,000 in molecular weight. In the presence of phosphoribosyl pyrophosphate, the enzyme was extremely heat-stable. The enzyme displayed Michaelis-Menten kinetics with apparent Michaelis constants for hypoxanthine, guanine and phosphoribosyl pyrophosphate of 1.59, 20.4 and 72.6 microM respectively.
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