Year-end Sale % OFF 
Abstract
Fractionation of Plasmodium falciparum cellular extracts by fast protein liquid chromatography (FPLC) identified at least two different DNA polymerases. An aphidicolin-sensitive activity co-purified with a primase activity. This, in combination with other characteristics (processivity, sensitivity to other inhibitors), most likely classifies this enzyme as an α-like DNA polymerase. It was, however, relatively resistant to N 2-( p- n-butylphenyl)deoxyguanosine 5′-triphosphate (IC 50 = 6.6 μM) and differs in this aspect from the host homologue, possibly indicating structural differences between host and parasite DNA polymerase α. The other DNA polymerase matched eukaryotic DNA polymerase γ in all properties tested.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
