Purification and characterization of cysteine protease from miswak Salvadora persica

Wesam H. Abdulaal

doi:10.1186/s12858-018-0100-1

Abstract

BackgroundGenerally, proteases in medicinal plants had different therapeutic effects such as anti-inflammatory effect; modulate the immune response and inhibitory effect toward tumor growth. In this study, protease was purified and characterized from miswak roots, as medicinal plant and natural toothbrush.ResultsPhysical and chemical characterization of cysteine protease P1 were studied such as pH optimum (6.5), optimum temperature (50 °C), thermal stability (50 °C) and Km (3.3 mg azocasein/ml). The enzyme digested some proteins in the order of caseine > haemoglobin > egg albumin >gelatin > bovine serum albumin. Hg2+ had strong inhibitory effect on enzyme activity compared with other metal ions. Kinetic of inhibition for determination the type of protease was studied. Iodoactamide and p-Hydroximercuribenzaoic acid (p-HMB) caused strong inhibitory effect on enzyme activity indicating the enzyme is cysteine protease.ConclusionsThe biochemical characterization of this enzyme will be display the suitable conditions for using of this enzyme in toothpaste in the future and the enzyme may be used in other applications.

Highlights

Proteases in medicinal plants had different therapeutic effects such as anti-inflammatory effect; modulate the immune response and inhibitory effect toward tumor growth
Two chromatography columns were used for purification of protease from miswak
Protease P1 was chromatographed on Sephacry S-200 column (Fig. 2), where the specific activity and fold purification raised to 355 units/mg protein and 9.1 fold, respectively

Summary

Introduction

Proteases in medicinal plants had different therapeutic effects such as anti-inflammatory effect; modulate the immune response and inhibitory effect toward tumor growth. Protease was purified and characterized from miswak roots, as medicinal plant and natural toothbrush. Proteases has been characterized from plants such as pea roots [1], ginger [2], Euphorbia microsciadia [3] and sweet potato [4]. Plant Proteases had medicinal properties such as bromelain proteases which treated anticancer and osteoarthritis [5,6,7,8]. Other proteases from malian medicinal plants treated schistosomiasis [12]. Papaya proteases had pharmaceutical applications such as antitumorals, anti-inflammatory, wound healing and digestive disorder [13]. The partially purified protease from B. subtilis substantially dehaired cow skin [14]

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Discussion

Conclusion