Abstract
Two peptides with antioxidant activity were isolated from Pseudosciaena crocea proteins. Pseudosciaena crocea muscle was hydrolyzed with neutral protease to obtain Pseudosciaena crocea protein hydrolysates (PCPH). After ultrafiltration through molecular weight cut-off membranes of 10, 5 and 3 kDa and assessment of free radical scavenging ability, the fraction (PCPH-IV) with the highest antioxidant activity was obtained. Several purification steps, i.e., ion exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography, were applied to further purify PCPH-IV. Two antioxidant peptides with the amino acid sequences Ser-Arg-Cys-His-Val and Pro-Glu-His-Trp were finally identified by LC-MS/MS.
Highlights
Free radicals are important signaling molecules that play key roles in gene expression, cell division and other normal physiological processes
Different kinds of antioxidant peptides have been isolated from cereal protein [3,4], fish protein [5,6,7,8], seed protein [9,10], egg white protein [11] and other food-borne proteins [12,13]
We used different enzymes to prepare antioxidant peptides from Pseudosciaena crocea and demonstrated that peptides obtained by neutral protease digestion exhibited the strongest Molecules
Summary
Free radicals are important signaling molecules that play key roles in gene expression, cell division and other normal physiological processes. We used different enzymes to prepare antioxidant peptides from Pseudosciaena crocea and demonstrated that peptides obtained by neutral protease digestion exhibited the strongest Molecules. Chi et al [8] have purified three antioxidant peptides, the isolation of peptides from Pseudosciaena crocea has not yet been fully explored. Chi et al [8]ofhave three peptides, peptides haveantioxidant not been identified The objectives this purified study were to antioxidant prepare Pseudosciaena the isolation of peptides from. Crocea protein hydrolysates, evaluate their antioxidant properties and further purify and identify the many of the peptides. Pseudosciaena crocea protein hydrolysates, evaluate their antioxidant properties and further purify and identify theand antioxidant peptides
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