Abstract
ABSTRACTAn alkaline lipase strain of Pseudomonas aeruginosa HFE733 was isolated from soil samples of domestic waste. The alkaline lipase from P. aeruginosa HFE733 was purified and characterized. The enzyme was purified 9.97-fold by means of ammonium sulphate precipitation, Sephadex G-25 and diethylaminoethyl (DEAE) cellulose chromatography. Purified alkaline lipase protein was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the molecular mass was 51.0 kDa. The enzyme exhibited maximum activity at 40 °C and pH 8.5. The enzyme is stable at pH 7.0–8.5. It was remarkably activated by some metal ions and chemical reagents, such as Fe3+, Al3+, β-mercaptoethanol, cysteine, DL-dithiothreitol (DTT), Tween 80 and Triton X-100, but suppressed by sodium dodecyl sulfate (SDS) at 10 mmol/L. The P. aeruginosa HFE733 strain and lipase exhibited remarkable potential for biodegradation of oil and organics (measured as chemical oxygen demand (COD)). We demonstrated that it can be used for biodegradation of food wastewater from restaurants.
Highlights
Lipases known as triacylglycerol acylhydrolases (E.C. 3.1.1.3) are ubiquitous carboxylic ester hydrolases that can catalyze hydrolysis of the long chain triglycerides to fatty acids, diacylglycerol, monoacylglycerol and glycerol [1]
Nucleotide-nucleotide BLAST in NCBI results showed that strain HFE733 has more than 99% sequence similarity with the first hit of Pseudomonas aeruginosa
As a step in our study, we evaluated the effect of the P. aeruginosa HFE733 fermentation broth in oil-rich restaurant wastewater treatment
Summary
Lipases known as triacylglycerol acylhydrolases (E.C. 3.1.1.3) are ubiquitous carboxylic ester hydrolases that can catalyze hydrolysis of the long chain triglycerides to fatty acids, diacylglycerol, monoacylglycerol and glycerol [1]. In non-aqueous medium, lipases synthesise esters from glycerol and long-chain fatty acids [2]. Lipases are largely produced from bacterial strains like Pseudomonas alcaligenes [3], Pseudomonas aeruginosa [4,5,6,7], Pseudomonas fragi [8], Bacillus subtilis [9] etc. More and more food wastewater is discharged in China, causing increasingly serious environmental pollution. Microbial strains and lipases could have an impact on reducing the fats and oil content in food wastewater, and may help in reducing severe pollution problems caused by wastewater
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