Abstract
Chitosanases play an important role in chitosan degradation, forming enzymatic degradation products with several biological activities. Although many chitosanases have been discovered and studied, the enzymes with special characteristics are still rather rare. In this study, a new chitosanase, CsnM, with an apparent molecular weight of 28 kDa was purified from the marine bacterium Pseudoalteromonas sp. SY39. CsnM is a cold-adapted enzyme, which shows highest activity at 40 °C and exhibits 30.6% and 49.4% of its maximal activity at 10 and 15 °C, respectively. CsnM is also a thermo-tolerant enzyme that recovers 95.2%, 89.1% and 88.1% of its initial activity after boiling for 5, 10 and 20 min, respectively. Additionally, CsnM is an endo-type chitosanase that yields chitodisaccharide as the main product (69.9% of the total product). It’s cold-adaptation, thermo-tolerance and high chitodisaccharide yield make CsnM a superior candidate for biotechnological application to produce chitooligosaccharides.
Highlights
Chitosan, a natural cationic polysaccharide, is a linear heteropolymer made of β (1–4) linkedNacetyl-D-glucosamine (GlcNAc or A) and D-glucosamine (GlcN or D)
Chitosanase (EC.3.2.1.132) is a type of glycoside hydrolase (GH) that catalyzes the hydrolysis of β-1,4-linked glycosidic bond, releasing chitooligosaccharides (CHOS) as main products
In the Carbohydrate-Active Enzymes database (CAZy), chitosanases are classified into six glycoside hydrolases (GH) families: 5, 7, 8, 46, 75, and 80 [18]
Summary
A natural cationic polysaccharide, is a linear heteropolymer made of β (1–4) linked. Nacetyl-D-glucosamine (GlcNAc or A) and D-glucosamine (GlcN or D). It is produced by the deacetylation of chitin which is the second-most ubiquitous polysaccharide in nature [1,2,3]. Chitosanase (EC.3.2.1.132) is a type of glycoside hydrolase (GH) that catalyzes the hydrolysis of β-1,4-linked glycosidic bond, releasing chitooligosaccharides (CHOS) as main products. The chemical structures and molecular sizes of the hydrolase products have a significant impact on these biological activities. In the Carbohydrate-Active Enzymes database (CAZy), chitosanases are classified into six glycoside hydrolases (GH) families: 5, 7, 8, 46, 75, and 80 [18]. Industrial production often requires enzymes with special properties, such as cold-adaptation, thermo-tolerance and single
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