Purification and characterisation of 1-dodecanol from an isolate of Streptomyces viridodiastaticus

Abstract

One-dodecanol was identified as the predominant secondary metabolite produced by a novel isolate of Streptomyces viridodiastaticus . A total secondary metabolite extract was electrophoresed, along with a prestained protein ladder (from 10 to 180 kDa), in a 10% SDS-polyacrylamide gel. Two prominent bands obtained were aligned with 25 and 10 kDa bands of the protein marker. These two secondary metabolite bands were also visualized by thin layer chromatography. The two bands, lower band (C1) and upper band (C2), in the polyacrylamide gel were sliced off and their metabolite content was eluted in n -butanol. Elution samples were analysed by GC-MS. Results indicated that the lower C1 band mostly contained 1-dodecanol, approximately 93%. The lower band also contained a 1-dodecanol-like metabolite, approximately 55%. The elution samples were also analysed by FTIR and the results were in accordance with those of the GC-MS. • The existence of 1-dodecanol as a secondary metabolite of Streptomyces viridodiastaticus was reported for the first time. • SDS-PAGE was also adopted for the first time in the resolution and purification of 1-dodecanol. • Results indicated that this approach could purify 1-dodecanol more than 90% (approximately 93%).