Abstract
A high purity of maize dwarf mosaic virus strain B (MDMV-B) with sufficient yield was obtained by isopycnic centrifugation in cesium sulphate. The virus was infectious and nonaggregated after the purification process. Ultraviolet absorption spectrum of the purified virus showed a typical curve of nucleroprotein with a maximum at 259 and a minimum at 249 nm. The yield of purified virus was 70-80μg per g of tissue. Antiserum to MDMV-B was produced by intramuscular injection of the purified virus to a rabbit. The highest titer of the antiserum obtained 7 weeks after the first injection was 1/8 in SDS-immunodiffusion test, and 1/2048 in precipitin ring test. The completely negative reactions of MDMV-B antiserum to healthy plant extract indicated the efficiency of this method for the purification of MDMV-B. A spur was produced between antigens of an isolate of sugarcane mosaic virus (SCMV) and MDMV-B against antiserum of MDMV-B in SDS-immunodif fusion test. Using the antiserum prepared in this study, we could easily distinguish MDMV-B from strains of SCMV by either SDS-immunodiffusion test or ELISA.
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