Puree and Juice of Thai Mango and Pineapple Analyzed by High-Performance Thin-Layer Chromatography Hyphenated with Effect-Directed Assays.

Gertrud E Morlock,Newitchaya Wutthinithisanand,Doris Rauhut

doi:10.3390/molecules26247683

Gertrud E Morlock, Newitchaya Wutthinithisanand + Show 1 more

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https://doi.org/10.3390/molecules26247683

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Abstract

The requirements for analytical tools are changing due to the global production chain, the increasing cases of adulteration, and the growing trend towards consumption of plant-based food products worldwide. The assessment of bioactivity of natural foods is currently not a quality criterion, and a paradigm shift is postulated. A non-targeted effect-directed profiling by high-performance thin-layer chromatography hyphenated with five different effect-directed assays was developed exemplarily for the puree and juice products of mango Mangifera indica L. (Anacardiaceae) and pineapple Ananas comosus (L.) Merr. (Bromeliaceae). Several bioactive compounds were detected in each sample. The additional bioactivity information obtained through effect-directed profiles improves, expands and modernizes product control. Non-target effect-directed profiling adds a new perspective to previous target analysis results that can be used not only to ensure health claims based on bioactive compounds, but also to detect unknown bioactive compounds coming from contamination or residues or changes caused by food processing.

Highlights

Bioactive compounds present in natural plant-based foods and their products attract increasingly both consumers and researchers
The total sugar of pineapple was higher than that reported by Lu et al [13]; values of natural plant-based food can vary depending on harvest time, climate influence each year, agricultural and soil management, among many other aspects
It can be used with regard to distinct health claims, e.g., based on antioxidative compounds

Summary

Introduction

Bioactive compounds present in natural plant-based foods and their products attract increasingly both consumers and researchers. Pineapple is considered a very nutritious and functional fruit, as it is rich in vitamin A, vitamin B, and minerals such as calcium, phosphorus and iron [10]. It contains several proteinases, such as bromelain, comosain and ananain, which support the digestive tract [11]. It has several compounds with antioxidant properties, namely ascorbic acid, β-carotene and phenolic compounds such as flavonoids [12,13,14,15]

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