Abstract
Objective To explore the therapeutic effect and mechanism of punicalagin on bronchial asthma in the elderly. Methods Peripheral venous blood was collected from healthy people and elderly patients with bronchial asthma. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured. PBMCs in the patient group were treated with different concentrations (0, 50, 100, and 200 mg/L) of punicalagin (PUN). MTT assay was used to detect cell activity, ELISA was used to detect the levels of IFN-γ, IL-2, IL-4, and IL-5, and Western blotting was used to detect the protein levels of p-STAT6, Jagged1, and GATA3. Result MTT results showed that 50–200 mg/L PUN had no cytotoxicity to PBMCs within 24 h. ELISA results showed that the levels of IFN-γ and IL-2 in the serum of the patients were significantly lower than those of healthy people, and the levels of IL-4 and IL-5 were significantly higher than those of the healthy people. PUN treatment significantly increased the levels of IFN-γ and IL-2 in the supernatant of PBMCs culture, while significantly decreased the levels of IL-4 and IL-5, and the change was proportional to the concentration of PUN. Western blotting results showed that the levels of p-STAT6, Jagged1, and GATA3 protein in PBMCs of patients were significantly higher than those of the healthy people. PUN treatment could significantly reduce the expression of p-STAT6, Jagged1, and GATA3 protein in PBMCs of patients, and the reduction level was proportional to PUN concentration. Conclusion PUN can inhibit Th2 differentiation and regulate Th1/Th2 balance by regulating IL-4/STAT6 and Jagged1/Notch pathways to alleviate the progress of bronchial asthma in the elderly.
Highlights
Bronchial asthma, known as asthma, is a chronic airway inflammation involving eosinophils, mast cells, lymphocytes, neutrophils, and other cells and various media, which is common to the elderly [1]. is chronic inflammation is associated with airway hyperresponsiveness, characterized by wheezing, chest tightness, shortness of breath and cough, and reversible episodes [2]
30 elderly patients with bronchial asthma admitted to our hospital from March 2021 to November 2021 were selected as the observation group, and 30 healthy people in the same period were selected as the control group
Compared with the control group, each concentration of PUN had no significant effect on the activity of Peripheral blood mononuclear cells (PBMCs), indicating that 50–200 mg/L PUN had no cytotoxicity to PBMCs within 24 h (Figure 1)
Summary
Known as asthma, is a chronic airway inflammation involving eosinophils, mast cells, lymphocytes, neutrophils, and other cells and various media, which is common to the elderly [1]. is chronic inflammation is associated with airway hyperresponsiveness, characterized by wheezing, chest tightness, shortness of breath and cough, and reversible episodes [2]. Known as asthma, is a chronic airway inflammation involving eosinophils, mast cells, lymphocytes, neutrophils, and other cells and various media, which is common to the elderly [1]. Due to the elderly patients with physical function is constantly weakening, immune ability is declining, respiratory system vulnerable leads to respiratory tract infection, and cause bronchial asthma, if not timely treated, will affect their health. Often occurs at night, prone to complications, severe and even lead to lung infection, and even respiratory failure to death. The imbalance of 1/ 2 in CD4+ cell subsets is currently recognized as the immunological pathogenesis of asthma [4]. Studies have shown that patients with asthma have excessive differentiation of 2 cells and excessive secretion of 2 cytokines [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
