Abstract
Pulsed field gel electrophoresis (PFGE) is an important technique for the separation of large DNA molecules by applying an electric field that periodically changes direction to a gel matrix. In 1984, Schwartz and Cantor described PFGE, introducing a new concept to separate DNA fragments larger than 50 kilobase pairs (kb). Then, a number of instruments based on this principle have been developed, and the value of using pulsed fields has been demonstrated for separating DNA fragments from a few kilobase pairs to over 10 megabase pairs (Mb). Over the past couple of decades, this technique has brought significant changes in fields of genetic research, such as comparative genomics of bacteria. In the current era of super high-throughput sequencing, PFGE still plays important roles in genomic analysis.
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