Abstract
A technique of pulsed low-energy electron holography is introduced, which allows for recording highly resolved holograms within reduced exposure times. Therefore, stacks of holograms are accumulated in a pulsed mode with individual acquisition times as short as 50 μs. Subsequently, these holograms are aligned and finally superimposed. The resulting holographic record reveals previously latent high-order interference fringes and thereby pushing interference resolution into the sub-nanometer regime. In view of the non-damaging character of low-energy electrons, the method is of particular interest for structural analysis of fragile biomolecules.
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