Abstract
Electrical stimulation is a well-known strategy for regulating cell behavior, both in pathological and physiological processes such as wound healing, tissue regeneration, and embryonic development. Electrotaxis is the directional migration of cells toward the cathode or anode when subjected to electrical stimulation. In this study, we investigated the conditions for enhanced directional migration of electrically stimulated adipose-derived stem cells (ADSCs) during prolonged culture, using a customized agar-salt electrotaxis chamber. Exposure of ADSCs to a 1200 μA electric current for 3 h, followed by cessation of stimulation for 6 h and resumed stimulation for a further 3 h, increased directional cell migration toward the anode without inducing cell death. Moreover, Golgi polarization maintained the direction of polarity parallel to the direction of cell movement. Herein, we demonstrated that a pulsed electric current is sufficient to trigger directional migration of ADSCs in long-term culture while maintaining cell viability.
Highlights
Cell migration is a fundamental process whereby cells are able to alter their position and move to the appropriate locations in order to execute their biological functions
As Adipose-derived stem cells (ADSCs) are located in adipose tissue and present no ethical and political issues compared to embryonic stem cells
We investigated cell damage caused by electrical stimulation (ES) of ADSCs in accordance with electrical exposure time
Summary
Cell migration is a fundamental process whereby cells are able to alter their position and move to the appropriate locations in order to execute their biological functions. This process is essential for proper development and maintenance of bodily tissues, as well as for the success of cell therapy [1,2,3]. ADSCs, compared to MSCs from other sources, have the highest proliferation ability, and retain their pluripotency after a long culture period These characteristics make ADSCs a more acceptable solution for tissue engineering applications in the field of regenerative medicine and clinical studies [8,9,10]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
