Abstract
The free radical scavenging activities of puerarin towards nitrogen dioxide radicals (NO2.), nitric oxide radicals (NO.) and hydroxyl radicals (OH.) were investigated by pulse radiolysis. Puerarin reacted With NO2. or NO. to form puerarin-4-O-. with the absorption maximum at 340 nm, but reacted with OH. to form at least three transient species including puerarin-2-O-. puerarin-4-O-. and [puerarin-OH](.) with a broad absorption spectrum band (300-750 nm). In addition, the reaction rate constants of puerarin reacting with NO2., NO. and OH. were determined as 2.6X 10(8), 1.7 X 10(8) and 3.9 X 10(9) L . mol(-1) . s(-1), respectively.
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