Pulse radiolysis of catalase in solution—II. Reactions of primary products from water radiolysis with catalase

Abstract

The mechanism of the reaction of catalase with e - aq, H and OH has been studied by pulse radiolysis at room temperature. Some evidences have been found that e - eq/H react with porphyrin ring of catalase to form π-radical without reduction of heme iron within investigated time span of 1 s after the pulse. OH radicals react mainly with the protein moiety of the enzyme but the formation and decay of compound I, an intermediate of the catalytic reaction of catalase can be observed as well.