Abstract
The aim of the present study was to evaluate histologically the pulp response of anionic lyophilized collagen matrix with or without hydroxyapatite as a biomaterial pulp-capping agent in pulpotomy of dogs' teeth. Sixty pre-molar roots from three dogs were used. After pulpotomy, the remaining pulp tissue was capped with one of the following materials: Group I (20 roots): anionic lyophilized collagen matrix; Group II (20 roots): anionic lyophilized collagen matrix associated with hydroxyapatite; Group III (10 roots): calcium hydroxide (p.a.) paste in saline; Group IV (10 roots): zinc oxide eugenol cement. After 90 days the animals were killed by anesthetic overdose and the teeth were removed and submitted to histological processing. According to the histopathological results, we concluded that the zinc oxide eugenol cement and anionic lyophilized collagen matrix with or without hydroxyapatite did not present satisfactory pulp response and that calcium hydroxide is the suitable material for pulpotomy.
Highlights
Pulpotomy is a conservative treatment of the radicular pulp tissue used in deciduous and permanent teeth consisting of the removal of inflammatory coronal pulp tissue at the entrance of the root canal and subsequent placement of a material to protect the radicular remnant
We evaluated the pulp response after protection with a collagen matrix associated or not with hydroxyapatite in pulpotomies in dogs’ teeth
The wide use of collagen in the field of biomaterials is associated to natural properties that include the low immune response, even when from heterologous sources, low toxicity, the ability to promote cellular growth and attachment, homeostasis[30] and the ability of collagen solutions to reconstitute in vitro into the microfibrillar structure found in natural tissues
Summary
Pulpotomy is a conservative treatment of the radicular pulp tissue used in deciduous and permanent teeth consisting of the removal of inflammatory coronal pulp tissue at the entrance of the root canal and subsequent placement of a material to protect the radicular remnant. This should preserve vitality of this tissue and stimulate the formation of mineralized tissue restoring pulp tissue function and normal structure. New biomaterials are being tested in vitro and in vivo for pulp protection in pulpotomies, most notably the bone morphogenetic proteins (BMPs), osteogenic proteins (OPs), transformation growth factor β (TGF-β) and collagen[4,5,6,7,8]. Some advantages of the use of collagen are the low immunogenic response[13,14], availability and abundance in nature, homeostatic effect[15,16], easy manipulation in different shapes and capacity to form fibers from soluble preparations
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