Abstract
The most common main materials for dentin bonding for composite resin restoration is 2-hydroxyethyl methacrylate (HEMA). HEMA has beneficial physical and chemical properties, and stable, yet toxic. The addition of ethanol or water, may reduce the toxic effect of HEMA. Ethanol solvent has lower H-bonding capacity compared to water solvent, so it can bind less free radicals from the residual monomer. This study aimed to analyze apoptosis due to dentine bonding application with ethanol and water solvent. Fibroblast culture cells were obtained from extracted third molar, by means of tripsinasion method. The cells were divided into 4 groups as reached confluent: cell culture without treatment as control, cell culture with scaffold chitosan, cell culture with scaffold and polymerized dentin bonding with ethanol or water solvent. Apoptosis observation was conducted using immunohistochemistry method with ethidium bromide acridin orange staining, under fluorescent microscope with 40´ magnification. There was a significant difference among groups (p=0.0001), yet no differences found between different solvent. Apoptosis rate in fibroblast cells culture exposed to HEMA bonding with ethanol solvent was 67%, while the cells exposed to HEMA bonding with water solvent was 44%. The effect of dentin bonding with ethanol solvent and water solvent towards apoptosis rate of pulp fibroblast cells is not different.
Highlights
Composite resin is restoration material that is commonly used in conservative dentistry for its esthetic value is better compared to other restoration material
The purpose of this study was to investigate the effect of apoptosis on fibroblast pulp cells after dentin bonding with hydroxyethyl methacrylate (HEMA) based on ethanol and water
Pulp Fibroblast Cell Isolation Pulp fibroblast cell culture was obtained from mandibular third molar of 18-years-old female patient that were extracted for orthodontic purpose, by means of explant method
Summary
Composite resin is restoration material that is commonly used in conservative dentistry for its esthetic value is better compared to other restoration material. Composite resin binds to teeth structure through adhesive material called dentin bonding. Most of various dentin bonding that is currently available, far, containing 2-hydroxyethyl methacrylate (HEMA) as basic material [1]. HEMA is widely used since have several advantages, such as having good physical-chemical properties, that is stable, both as base material bonding and when mixed with other materials [1]. Based on in vitro study, HEMA can stimulate apoptosis after 24 h [3]. HEMA can induce the production of Reactive Oxygen Species (ROS) in fibroblast cells that cause DNA fragmentation, characterized by apoptosis due to oxidative stress [4]
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