Pulmonary absorption of aerosolized fluorescent markers in the isolated rabbit lung

Abstract

For the development of aerosolized controlled release formulations such as liposomes or nanoparticles, the use of suitable model drugs is necessary. This study compared the pulmonary absorption of the three structurally diverse fluorescent markers 5(6)-carboxyfluorescein (CF), 8-methoxypyrene-1,3,6-trisulfonic acid trisodium salt (MPTS) and rhodamine 6G (R6G) after nebulization in an isolated, perfused and ventilated rabbit lung. Aerosol particle size and lung deposition as well as lipophilicity of the fluorescent markers were determined. Dye concentrations were measured in the recirculating buffer and in the bronchoalveolar lavage. The MMAD of the dye aerosols ranged between 4.70 and 4.88 μm, total lung deposition was 0.40 ± 0.05 ml. The 1-octanol/water partition coefficient as measure for lipophilicity was −3.45 ± 0.16 for CF, −4.95 ± 0.21 for MPTS and 2.69 ± 0.18 for R6G. The perfusate concentration showed an increase to ∼400 ng/ml (53.4 ± 6.8% of the intrapulmonary deposited dye) for CF and ∼230 ng/ml (29.1 ± 2.0%) for MPTS, respectively; R6G concentration increased in the first 30 min to ∼38 ng/ml followed by a gradual decrease to ∼26 ng/ml (3.3 ± 0.7%). In conclusion, these data suggest that the hydrophilic dye CF is suitable to study drug transport from aerosolized controlled release formulations across the lung barrier. In contrast, the highly water-soluble fluorescent probe MPTS demonstrates insufficient recovery and the lipophilic R6G high accumulation in lung tissue.