Abstract
The formation is described of a extracellular polysaccharide by a yeast-like fungus, morphologically identical with Aureobasidium pullulans syn. Pullularia pullulans. The polymer is formed in a yield of 20 to 22% in Czapek-Dox medium with glucose, saccharose and fructose as a source of carbon. Thiamine has a stimulating effect increasing the yield to 32%. However, the production during optimal growth on maltose was low. The polymerisation product has a optical activity [ α] D 20 in H 2O of + 168°. Chromatography showed glucose to be present as sole product after hydrolysis. After partial hydrolysis isomaltose and a small percentage of maltose were identified. The infra-red spectrum suggested a predominant percentage of α-I,4-linkages. The similarity to bacterial dextrans is discussed.
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