Puerarin Inhibits Adhesion Molecule Expression in TNF-α-Stimulated Human Endothelial Cells via Modulation of the Nuclear Factor ĸB Pathway

Abstract

Background: The isoflavone puerarin is the most abundant isoflavone-C-glucoside extracted from the root (radix puerariae) of the plant Pueraria lobata and possesses many biological activities. In this report, the ability of puerarin to modulate intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1) and endothelial leukocyte adhesion molecule 1 (E-selectin), and to induce changes in the nuclear factor ĸB (NF-ĸB) pathway in human umbilical vein endothelial cells (HUVECs) was examined. Methods: The protein and mRNA levels of tumor-necrosis-factor-α (TNF-α)-induced ICAM-1, VCAM-1 and E-selectin were determined in HUVECs. Inhibitor ĸB (I-ĸB) phosphorylation and p65 NF-ĸB expression in HUVECs were also examined. Results: Puerarin inhibited the expression of TNF-α-induced ICAM-1, VCAM-1 and E-selectin proteins and mRNAs in HUVECs. Subsequently, we determined that the inhibition of ICAM-1, VCAM-1 and E-selectin expression was due to a dose-dependent suppression of phosphorylation and degradation of I-ĸB, which resulted in a reduction of p65 NF-ĸB nuclear translocation. Conclusion: These data suggested that the effect of puerarin-mediated inhibition of TNF-α-induced ICAM-1, VCAM-1 and E-selectin expression is attributed to suppressed NF-ĸB activation on the transcriptional level.