Abstract
Out of 50 samples were taken from each carcass of camel, cattle, sheep and goat from Kalyobia province abattoirs, the incidence rates of enterohaemorragic Escherichia coli O157: H7 strain were 6, 8, 11 and 9 with the percentage of 12%, 16%, 22% and 18% respectively. In the same time out of 50 samples were taken from diarrheic human in the same localities of animals, the incidence rate of enterohaemorragic Escherichia coli O157: H7 strain was 22 with the percentage of 44%. Isolates were tested for susceptibility to 6 antimicrobial drugs by discs (AMP, ampicillin; NAL, nalidixic acid; CIP, ciprofloxacin; CHL, chloramphenicol; TET, tetracyclin; TRI, trimethoprim) diffusion and agar dilution methods in order to determine minimum inhibitory concentrations (MIC). Multiplex PCR amplification was used to detect genes conferring resistance to: ampicillin (TEM and SHV beta-lactamase), chloramphenicol (catI, catII, catIII and cml), tetracycline (tetA, tetB, tetC, tetD, tet E and tetG), and trimethoprim (dfrA1, dfrA9, dfrA12, dfrA13, dfr7, and dfr17).
Highlights
Bacterial resistance to antimicrobials is a global problem, and understanding the molecular basis of resistance acquisition and transmission can contribute to the development of new strategies to combat this phenomenon
In this study we isolated and identified Escherichia coli O157:H7 serotypes from some animals carcasses and diarrheic human in the same localities at Kalyobia province, the samples were taken from carcasses of camel; cattle, sheep and goat at Kalyobia province abattoirs, in the same manner human samples were taken from individual case occupational contact with these animals
From this work we conclude that E. coli O157:H7 infections were directly transmitted from animals and their environment to people
Summary
Out of 50 samples were taken from each carcass of camel, cattle, sheep and goat from Kalyobia province abattoirs, the incidence rates of enterohaemorragic Escherichia coli O157: H7 strain were 6, 8, 11 and 9 with the percentage of 12%, 16%, 22% and 18% respectively. Same localities of animals, the incidence rate of enterohaemorragic Escherichia coli O157: H7 strain was 22 with the percentage of 44%. Isolates were tested for susceptibility to 6 antimicrobial drugs by discs (AMP, ampicillin; NAL, nalidixic acid; CIP, ciprofloxacin; CHL, chloramphenicol; TET, tetracyclin; TRI, trimethoprim) diffusion and agar dilution methods in order to determine minimum inhibitory concentrations (MIC). Multiplex PCR amplification was used to detect genes conferring resistance to: ampicillin (TEM and SHV betalactamase), chloramphenicol (catI, catII, catIII and cml), tetracycline (tetA, tetB, tetC, tetD, tet E and tetG), and trimethoprim (dfrA1, dfrA9, dfrA12, dfrA13, dfr, and dfr17)
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