Abstract
Much of the cost associated with marker discovery for marker assisted breeding (MAB) can be eliminated if a diverse, segregating population is generated, genotyped, and made available to the global breeding community. Herein, we present an example of a hybrid, wild-derived family of the octoploid strawberry that can be used by other breeding programs to economically find and tag useful genes for MAB. A pseudo test cross population between two wild species of Fragaria virginiana and F. chiloensis (FVC 11) was generated and evaluated for a set of phenotypic traits. A total of 106 individuals in the FVC 11 were genotyped for 29,251 single nucleotide polymorphisms (SNPs) utilizing a commercially available, genome-wide scanning platform (Affymetrix Axiom IStraw90TW). The marker trait associations were deduced using TASSEL software. The FVC 11 population segregating for daughters per mother, inflorescence number, inflorescence height, crown production, flower number, fruit size, yield, internal color, soluble solids, fruit firmness, and plant vigor. Coefficients of variations ranged from 10% for fruit firmness to 68% for daughters per mother, indicating an underlying quantitative inheritance for each trait. A total of 2,474 SNPs were found to be polymorphic in FVC 11 and strong marker trait associations were observed for vigor, daughters per mother, yield and fruit weight. These data indicate that FVC 11 can be used as a reference population for quantitative trait loci detection and subsequent MAB across different breeding programs and geographical locations.
Highlights
The expense of developing a dense linkage map of single sequence repeats (SSRs) (Sargent et al, 2012) and/or single nucleotide polymorphism (SNPs) (Bassil et al, 2015) to conduct marker assisted breeding (MAB) (Collard and Mackill, 2008) is prohibitive for many breeding programs, in the developing countries
We describe a hybrid population of the octoploid strawberry that should be a rich source of novel genes for the global breeding community
The F. virginiana had the highest value for vigor, crown production, inflorescence number, inflorescence height, flower numbers, yield, and depth of fruit color, while the F. chiloensis had the highest values for fruit weight and firmness
Summary
The expense of developing a dense linkage map of single sequence repeats (SSRs) (Sargent et al, 2012) and/or single nucleotide polymorphism (SNPs) (Bassil et al, 2015) to conduct marker assisted breeding (MAB) (Collard and Mackill, 2008) is prohibitive for many breeding programs, in the developing countries. Much of this cost can be circumvented; if a diverse, segregating population is made available to the global breeding community that has already been genotyped using markers from a published linkage map. Costs could be further reduced by utilizing publicly available association mapping software such as ‘Tassel’ (Bradbury et al, 2007; Khan, 2011)
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