Abstract
Seven new triterpenoid saponins (1–7), together with three known ones (8–10), were isolated from Ilex pubescens. Elucidation of their structures was performed based on high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS), infrared spectra (IR), and nuclear magnetic resonance (NMR) spectroscopic data. The anti-inflammatory activity of the isolates toward lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages was investigated. The results demonstrated that compounds 3, 5, and 6 inhibited the expression of inducible nitric oxide synthase (iNOS) protein in comparison with LPS stimulation in RAW264.7 cells.
Highlights
Ilex pubescens, belonging to the plant family Aquifoliaceae, is widely distributed throughout the south of China
Pubescenoside E (1) (Figure 1) was isolated as a white amorphous powder, and the molecular formula was deduced to be C47 H74 O17 based on the quasi-molecular ion peak [M + COOH]− at m/z
nuclear magnetic resonance (NMR) spectra, we identified the structure of compound
Summary
Ilex pubescens, belonging to the plant family Aquifoliaceae, is widely distributed throughout the south of China. The primary components that have been reported from this plant include triterpenoids [4], phenolic glycosides [5], lignan glycosides [6], hemiterpene glycosides [7], and flavonoids [8]. All of the components described above play an important role related to several bioactivities, such as anti-inflammatory [9,10,11,12], anticoagulant [13], and antithrombotic activities [14]. Lipopolysaccharide (LPS)-induced macrophages are widely used to study inflammatory responses in vitro [15]. Macrophages are versatile cells that play many roles, and can be activated by external stimuli, such as LPS, to release excessive amounts of inflammatory mediators, including prostaglandin E2
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
