PUB024 Real-Time Computed Tomography Fluoroscopy Guidance in a Rabbit Model of Solitary Lung Cancer

Abstract

We evaluate the feasibility and safety of a newly developed solitary lung cancer rabbit model that utilizes real-time computed tomography (CT) fluoroscopy-guided inoculation of VX2 single cell suspensions. Thirty-eight rabbits were divided into four groups according to number of VX2 carcinoma cells, Lipiodol amount, Matrigel amount, and injection needle size. The different VX2 tumor cell suspensions were percutaneously injected into rabbits under real-time CT fluoroscopy guidance. Two weeks later, VX2 lung cancers were confirmed by positron emission tomography/CT, necropsy, and histology. Real-time CT fluoroscopy allowed the precise inoculation of tumor cell suspensions containing Lipiodol. Use of Matrigel and a small-sized needle reduced spreading and leakage of tumor cell suspensions in the lung parenchyma. Solitary lung cancers were successfully established in all rabbits in group 4 (22/22, 100%); these rabbits were inoculated with 150 μl VX2 tumor cells filtered through a 100 μm cell strainer, 100 μl Lipiodol, and 150 μl Matrigel, using 26-gauge needles. Pneumothorax was observed in only 2 of 38 rabbits (5.3%).View Large Image Figure ViewerDownload Hi-res image Download (PPT) Real-time CT fluoroscopy-guided inoculation of the appropriate composition of a VX2 tumor cell suspension using a small sized needle is an easy and safe method to model solitary lung cancer in rabbits.