Abstract
This study investigates the expression of nonreceptor protein tyrosine phosphatase 6 (PTPN6) gene in different colon cancer cells and its effect on malignant biological behavior. The expression level of PTPN6 mRNA in different colon cancer cell lines was detected by qPCR. CCK-8, clone formation assay, scratch assay, and transwell assay were used to detect the effect of knockdown or overexpression of the PTPN6 gene on the malignant biological behavior of colon cancer cells. CO-IP assay was used to detect the interaction protein of PTPN6. PTPN6 was highly expressed in colorectal cancer tissues. High expression of PTPN6 is associated with poor prognosis in patients with colon cancer. PtPN6 knockdown inhibited the proliferation, invasion, migration, and clonogenesis of colorectal cancer LOVO and SW480 cells. At the same time, the knockdown of PTPN6 inhibited the EMT process in colorectal cancer. CO-IP results showed that PTPN6 had a protein-protein interaction with EGFR. Overexpression of EGFR increased the carcinogenic effect of PTPN6. The high expression of the PTPN6 gene can promote the proliferation, migration, and invasion of colon cancer cells. PTPN6 can interact with EGFR. PTPN6-EGFR complex may be an important factor affecting the biological characteristics of colon cancer cells and a potential therapeutic target.
Highlights
Colon cancer is the third most common malignancy in the world and the fourth most deadly [1,2,3]
Results of survival analysis showed that patients with high expression of PTPN6 had a short survival time. e expression of PTPN6 was associated with poor prognosis (Figure 1(b))
Immunohistochemical results showed that the expression of PTPN6 protein in colon cancer tissue was consistent with the expression trend of PTPN6 mRNA. e expression of PTPN6 protein was significantly increased in colon cancer tissues (Figure 1(c))
Summary
Colon cancer is the third most common malignancy in the world and the fourth most deadly [1,2,3]. Further study on the mechanism of colon cancer metastasis is of great significance to improve the prognosis of patients [6, 7]. Protein interaction networks are of great significance for the study of various signal networks and transcriptional regulation processes in living organisms [17]. Inhibition of PTPN6 aggravates alumina nanoparticle-induced COPD in mice by activating the STAT pathway [21]. E epidermal growth factor receptor (EGFR) signaling pathway is usually closed in normal tissues but activated and opened in tumor tissues. Ese results indicate that the EGFR signaling pathway is involved in the regulation of tumor development. We detected the expression of PTPN6 in different colon cancer cell lines. E purpose of this study is to provide new ideas for the treatment of colon cancer The interaction protein of PTPN6 was studied. e purpose of this study is to provide new ideas for the treatment of colon cancer
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