Abstract
Hepatocellular carcinoma (HCC) is a life-threatening malignant tumor with high cancer mortality, therefore it is particularly important to sensitively detect the biomarkers of hepatocellular carcinoma for early diagnosis and treatment. In this work, a nonenzymatic and ultrasensitive electrochemiluminescence (ECL) cytosensor for HepG2 cells detection is established based on the PtNi nanocubes-catalyzed tyramine signal amplification. PtNi nanocubes (PtNi NCs) are firstly reported to catalyze tyramine-luminol (Tyr-Lum) with the aid of H2O2 for covalently binding to membrane proteins of HepG2 cells, realizing the introduction of massive Tyr-Lum ECL luminophores for signal amplification. Besides, PtNi NCs could also enhance the ECL signal of luminol-H2O2 system. On the basis of bifunctional PtNi NCs, the developed ECL cytosensor for HepG2 cells detection shows a wider linear range of 1 × 10 to 1 × 105 cells mL−1 with detection limit of 3 cells mL−1. This proposed enzyme-free-mediated tyramine signal amplification inspires a new strategy for applications in the early diagnosis of cancer cells.
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