Abstract
Renal ischemia-reperfusion (I/R) injury, one of the drastic outcomes of renal failure and organ transplantation, tends to deteriorate over time; therefore, noninvasive therapeutic strategies will avail the progression-free survival of the patients. Magnetic field has been proposed as a noninvasive treatment strategy; however, with recent scientific advances, many controversies have arisen regarding its efficacy. Pterostilbene, a natural analog of resveratrol, was documented to be effective in treatment of I/R injuries. This study aims to assess the acute therapeutic effects of combined extremely low-frequency magnetic field (ELF-MF) and pterostilbene treatment on renal I/R injury. After induction of renal I/R in Wistar rats, treatments of 50 Hz, 1 mT ELF-MF applied alone or in combination with pterostilbene were applied for 5 consecutive days. Kidney homogenates were analyzed by Fourier transform infrared spectroscopy. I/R injury resulted in an altered protein and lipid structure with the dominance of longer acyl chains; a slight decrease in lipid, protein, unsaturated lipid, and unsaturated/saturated lipid content; and an increase in membrane fluidity and lipid peroxidation in rat kidneys. Although ELF-MF treatment alone was not sufficient to restore all ischemia-induced alterations, the combined treatment strategy of pterostilbene administration in the presence of ELF-MF was successful and warrants further investigation.
Highlights
Acute renal ischemia-reperfusion (I/R) injury can be described as the damage occurring in ischemic renal cells after the reestablishment of the blood supply (Gao et al, 2018)
Discussion the effective role of Pte administration in preventing I/R injury was previously reported (Gao et al, 2018), the potential efficacy of extremely low-frequency magnetic field (ELF-magnetic field (MF)) in renal ischemic injury is still not clear, and there have been controversial results reported in the literature regarding the efficiency of ELF-MF
In an Fourier transform infrared (FTIR) spectrum, information on the concentration or content of a particular functional group can be acquired from band areas and/or signal intensities, information about structural alterations in macromolecules can be obtained by monitoring the frequency shifts of the bands, and bandwidth values can be used to monitor dynamic properties of various molecules in the system (Umemura et al, 1980; Bozkurt et al, 2010; Cakmak et al, 2011, 2012)
Summary
Acute renal ischemia-reperfusion (I/R) injury can be described as the damage occurring in ischemic renal cells after the reestablishment of the blood supply (Gao et al, 2018). The pathophysiology of acute renal failure has been closely connected to tubular epithelial cell injury. Pte is a wellrecognized antioxidant primarily found in blueberries and the heartwood of Pterocarpus marsupium (Leguminosae). It has traditionally been used for the treatment of diabetes in Indian medicine and has been shown to control blood sugar levels in experimental diabetic animals (McCormack and McFadden, 2013).
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