Abstract
The effects of folic acid and aminopterin were studied on the steroid 11β and 21-hydroxylases of domestic duck adrenals. Aminopterin partially inhibited the transformation of labelled, exogenous progesterone to 11β-, 18- and 21-hydroxylated metabolites by a duck adrenal gland whole homogenate, while the presence of folic acid resulted in a slight stimulation of the sequential hydroxylation of progesterone. When added to duck adrenal mitochondrial or microsomal preparations aminopterin effected a concentration dependent inhibition of the 11β- or 21-hydroxylase activity while the presence of folic acid resulted in a concentration dependent stimulation. Aminopterin inhibition of mitochondrial 11β-hydroxylase was competitive, while that of the microsomal 21-hydroxylation was non-competitive. Neither folic acid or aminopterin induced difference spectra of mitochondrial or microsomal cytochrome P-450.
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