Abstract
Noble metal nanoenzymes such as Pt, Au, Pd, etc. exhibit magnificent activity. However, due to the scarce reserves and expensive prices of precious metals, it is essential to investigate their enzyme-like activity and explore the possibility of their reuse. In this work, the oxidase-like activity and reusability of several Pt nanoparticles with different morphologies were detected. We compared the Pt nanoparticles (NPs) with a size of about 30 nm self-assembled by 5 nm Pt nanoparticles and Pt nanoparticles (Pt-0 HCl) with a diameter of about 5 nm, and found that their Michaelis−Menten constants (Km) were close and their initial performance similar, but the Pt NPs had better reusability. This was probably attributed to the stacked structure of Pt NPs, which was conducive to the substance transport and sufficient contact. At the same time, it was found that the size, dispersion, and organic substances adsorbed on the surface of Pt nanoparticles would have a significant impact on their reusability. A colorimetric detection method was designed using the oxidase-like activity of Pt NPs to detect ascorbic acid in triplicate. The limits of detection were 131 ± 15, 144 ± 14, and 152 ± 9 nM, with little difference. This research not only showed that the morphology of the catalyst could be changed and its catalytic performance could be controlled by a simple liquid phase synthesis method, but also that it had great significance for the reuse of Pt nanoenzymes in the field of bioanalysis.
Highlights
Ascorbic acid (AA), as an antioxidant, plays an important role in many biochemical and physiological processes, such as the formation of neurotransmitters, ion absorption, amino acid metabolism, and so on [1,2]
We found that Pt NPs, as synthesized, were reusable and could catalyze 3, 3, 5, 5 -tetramethylbenzidine (TMB) oxidation multiple times in a row
We explored a series of Pt NPs and found that their reusable properties and affinities to the substrate were strongly related to their morphology and surface state
Summary
Ascorbic acid (AA), as an antioxidant, plays an important role in many biochemical and physiological processes, such as the formation of neurotransmitters, ion absorption, amino acid metabolism, and so on [1,2]. Mainstream methods for detecting AA have been reported including the electrochemical method, liquid chromatography, fluorescence method, titration, and chemiluminescence technology [11,12,13] These methods all have the disadvantages of complicated and expensive operations. The method for colorimetric detection of AA was established to detect AA in triplicate, and the limits of detection were 131 ± 15, 144 ± 14, and 152 ± 9 nM, which had broad application prospects These investigations extend our understanding of the properties of Pt NPs, and provide a significant basis for the development and design of nanoenzymes
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