Abstract
A new glucose biosensor, based on the modification of highly ordered Au nanowire arrays (ANs) with Pt nanoparticles (PtNPs) and subsequent surface adsorption of glucose oxidase (GOx), is described. Morphologies of ANs and ANs/PtNPs were observed by scanning electron microscope. The electrochemical properties of ANs, ANs/GOx, ANs/PtNPs, and ANs/PtNPs/GOx electrodes were compared by cyclic voltammetry. Results obtained from comparison of the cyclic voltammograms show that PtNPs modification enhances electrochemical catalytic activity of ANs to H2O2. Hence, ANs/PtNPs/GOx biosensor exhibits much better sensing to glucose than ANs/GOx. Optimum deposition time of ANs/PtNPs/GOx biosensor for both amperometric and potentiometric detection of glucose was achieved to be 150 s at deposition current of 1 × 10−6 A. A sensitivity of 0.365 μA/mM with a linear range from 0.1 to 7 mM was achieved for amperometric detection; while for potentiometric detection the sensitivity is 33.4 mV/decade with a linear range from 0.1 to 7 mM.
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