Abstract
We report a sensitive and stable electrochemical impedance immunosensor prepared with functionalized Pt nanoparticles decorated graphene monolayer deposited on a glassy carbon electrode (GCE). Graphene monolayer was grown on a copper foil by chemical vapor deposition (CVD) method using acetylene as a precursor source and transferred it on GCE and electrochemically oxidized to electroactive graphene (EG). 3-Mercaptopropionic acid (MPA) functionalized Pt-nanoparticles, Pt (MPA), were attached to electroactive graphene (EG) using a molecular bilinker 1-pyrenemethylamine (PMA). Pt (MPA) NP offers free terminal carboxyl group for the site specific covalent biomolecular immobilization of troponin antibody, anti-cTnI, through carbodiimide coupling reaction to form an anti-cTnI-Pt(MPA)-PMA/EG/GCE bioelectrode. The bioelectrode was characterized by transmission electron microscopy, scanning electron microscopy, Raman spectroscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The bioelectrode exhibited a linear electrochemical impedance response to cTnI over the concentration range of 0.01–10ngmL−1 in phosphate buffer saline (pH 7.4) with a sensitivity of 80Ωcm2 per decade and a limit of detection of 4.2pgmL−1 cTnI.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
