Abstract
A lipase producer psychrophilic microorganism isolated from Arctic sample was studied. The genomic DNA of the isolate was extracted using modified CTAB method. Identification of the isolate by morphological and 16S rRNA sequence analysis revealed that the isolate is closely related to Arthrobacter gangotriensis (97% similarity). A. gangotriensis was determined as positive lipase producer based on the plate screening using specific and sensitive plate assay of Rhodamine B. The PCR result using Arthrobacter sp.'s full lipase gene sequence as the template primers emphasised a possible lipase gene at 900 bp band size. The gene is further cloned in a suitable vector system for expression of lipase.
Highlights
Psychrophiles are defined as organisms having an optimal temperature for growth at 15°C or lower with a maximum temperature for growth at about 20°C (Morita 1975)
A lipase producer psychrophilic microorganism isolated from Arctic sample was studied
A. gangotriensis was determined as positive lipase producer based on the plate screening using specific and sensitive plate assay of Rhodamine B
Summary
Psychrophiles are defined as organisms having an optimal temperature for growth at 15°C or lower with a maximum temperature for growth at about 20°C (Morita 1975). They produce enzymes which are adapted to work at low temperature. Previous studies have it have shown that cold-adapted microorganisms are potential sources of cold-active enzymes that can exhibit higher catalytic activity at low temperature (Gerday et al 2000; Giudice 2006). One of the enzymes produced by psychrophilic microorganisms includes lipases. Lipases normally catalyse heterogenous reactions at the interface of water and insoluble systems
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