PSXVI-15 Study of the content of maternal lymphocytes with Barr’s bodies in the bloodstream and red bone marrow of newborn male mice

Abstract

Abstract In animals with hemochorical placenta, the transfer of maternal antibodies and white blood cells to the fetus can take place along the transplacentary pathway. In the first hours of life, the intestines of newborn animals are able to absorb antibodies from colossus by pinocytosis. Similarly, maternal lymphocytes can get into the blood of a newborn. The aim of this work was to identify maternal lymphoid cells with sexual x-chromatin in the blood and red bone marrow of newborn male mice. Studies were conducted on nonlinean lactating mice and newborn male mice. Two groups of five animals were formed. The mice had breast glands. A longitudinal incision was made and a print smear was prepared. The bone marrow was taken from the proximal part of the femur. Blood was obtained by amputating the tip of the tail. All the drugs were stained by Pappenheim and examined with immersion optics. In the study of breast tissue smears, the number of lymphocytes in the microscope fields was counted. Visualization of drugs showed their high frequency of occurrence (15,1±1.7 in 100 fields of vision). In three-day male mice, the percentage of lymphocytes in the blood was 55.4±3.5%, including cells with x-chromatin (2.4±0.2±%). of which 5.3±3.2% were maternal cells with Barra Taurus. The appearance of maternal lymphocytes in the systemic circulation of newborn male mice is explained by the possibility of their transfer through the hemochorial placenta or absorption into colos dairy through the intestines. Active migration of maternal lymphocytes from the systemic circulation of young animals to the red bone marrow is necessary for the formation of cellular immunity in the body during the period of growth and development.