PSXIII-37 Alteration of mRNA abundance of the oxidizing factors-related genes during the transition period in dromedary camel

Abstract

Abstract Livestock near and during parturition have a complex defense system against environmental and physiological stresses. Little knowledge exists for some livestock species, such as camels, during this time. However, camels are an important species in some areas of the world. This study aimed to investigate the changes in the expression of genes responsible for the regeneration of hepatic oxidizing factors. Ten multiparous (2.38 ± 0.56; mean ± SEM parity) pregnant camels weighing 527.94 ± 73.46 kg BW were studied. Camels pastured daily, kept in individual stalls nightly with a partial mixed ration and free access to water while in stalls. Hepatic biopsies were taken days 60, 45, 30, and 15 antepartum (AP) and days 3, 15, 30, 45, and 60 postpartum (PP). The mRNA abundance of glutathione reductase (GSR1), thioredoxin reductase (TrxR1), and sulfiredoxin (Srx1) were determined using the qPCR technique. Significances were determined using the general linear model with repeated measures of IBM SPSS statistics software v. 21.0 and are presented as means ± SEM (Figure 1). Means differences were determined using LSD test and considered significant if P values < 0.05. Compared to d 60AP, mRNA abundance of TrxR1 and Srx1 reached their peak at d 3PP (P ≤ 0.03). Although TrxR1 decreased slightly by d 15 and 30PP compared to d 3PP (P = 0.04), it was higher compared to all AP time-points (P ≤ 0.05). mRNA abundance of Srx1 decreased at d 30PP compared to d 3PP (P = 0.05). In contrast, the frequency of the GSR1 mRNA was static until d 30PP, reduced slightly (P ≤ 0.05) then was unchanged to the end of the study period. These results show that genes responsible for hepatic oxidizing factors could be considered immunoregulatory factors for orchestrating cytoprotective systems against oxidative stress.