Abstract

Abstract The objective was to evaluate the use of guava extract (Psidium guajava L.) as an antioxidant additive in the diets of Japanese quails (Coturnix coturnix japonica) in laying phase and to verify the incubation parameters of eggs stored at different periods pre-incubation. The experiment was conduced at Federal University of Goias. The extract contained 2.13% ellagic acid. A four hundred and thirty-two (432) eggs of Japanese quails fed with guava extract in the diet were incubated in a completely randomized design, in a single-stage incubator. Temperature of 37°C and humidity of 65% was manteined. The eggs were distributed in 4x2 factorial design (levels of guava extract and stored pre-incubation period) with 54 replicates, each egg being considered an experimental unit. Four levels of guava extract were added in diets of the breeders (0.0%, 0.3%, 0.6% and 0.9%) and two stored pre-incubation period (three and nine days) were studied. The results were submitted to analysis of variance and Tukey test at 5% probability. To embryo diagnostic was used Fisher exact test. The eclosion percentage was not influenced by factors studied. There was a significant interaction (P < 0.05) between effects of levels of guava extract in diet and stored pre-incubation period on hatch window. The hatch window was shorter for quails from egg storage for nine days and breeders fed a 0.0% guava extract. The lower level of guava extract supplementation in diets (0.3%) of breeder promotes padronizing hatcheries time. The neonates quails from eggs storaged for nine days hatched in shorter period time in relation to quails from the eggs stored for three days. Values of the embryo diagnostic were not different (P > 0.05). It is concluded that the use of 0.3% inclusion of guava extract containing ellagic acid in layer quail diets promotes standardization of hatchery window of neonate quails.

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