Abstract
Abstract The aim of this study is to asset toxic influence of heavy metals in fish. This research was intended to analyze aminotransferases (ALT, AST) serum activity under the influence of various lead concentrations in European carp. Intracellular enzymes activity level elevation in serum is a sign of mass cell destruction and consequently pathological process. In this experiment 3 groups of fish (European carp) were formed, 10 fish in each group: control group; 1-experimental group – fish were contained in water with 0.06 mg/l lead acetate concentration (10 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation); 2-experimental group – fish were contained in water with 0.6 mg/l lead acetate concentration (100 times higher than fishery waters permissible exposure limit according to the laws of the Russian Federation). Exposure time was 4 hours. The blood samplings were taken from heart by syringe and placed in dry test tubes. After centrifugation of the samplings in non-hemolysed serum samples ALT and AST activities were assessed by standard methods. The study revealed that there were meaningful elevation of ALT and AST serum activity (P ≤ 0.001) by 43.8% (30.83±1.14 IU/l) and 17.58% (266.52±6.37 IU/l) accordingly in 1-experimental group in compare with control group (21.44±2.17 IU/l and 226.68±4.35 IU/l). In case where the lead acetate concentration were 100 times higher than fishery waters permissible exposure limit there were meaningful elevation of ALT and AST serum activity (P ≤ 0.001) by 74.86 % (37.49±2.76 IU/l) and 66.23% (376.82±19.45 IU/l) accordingly in 2-experimental group in compare with control group. After analyzing the received data there were established meaningful aminotransferases serum activity elevation under the influence of various lead concentrations in European carp. This is possibly connected to lipid peroxygenation processes disruption in muscles and liver under the lead toxic influence.
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