PSXI-16 Levels of a blend of clove, castor and cashew oils and microencapsulated active ingredients (eugenol, thymol and vanillin) on ruminal gram-positive bacteria population density

Abstract

Abstract This study was realized to evaluate de effect of a blend of vegetable oils and active ingredients (Ruminatus® - safeeds@safeeds.com.br) on ruminal population density of Ruminococcus albus, Ruminococcus flavefaciens and Streptococcus bovis. The blend contained clove (Eugenia caryophyllata), castor (Ricinus communis) and cashew (Anacardium occidentale) oils and microencapsulated active ingredients (eugenol, thymol and vanillin). The experiments were designed using each bacteria exposed to doses of 1.5; 3.0; 4.5 and 6.0 mg/ml of the product and with six replicates. Bacteria were cultivated on Hobson’s M2 medium in Hungate tubes. Cultures cultivated without the addition of any oils were considered as control. Bacteria were cultivated overnight and freshly inoculated into new tubes containing medium. The antimicrobial activity was evaluated using a Spectrophotometer Evolution 201 UV-visible (Thermo Scientific) at 600 nm. Readings were performed at 0, 8, 12 and 24 hours after inoculation. Data were analyzed using the linear mixed models and the NLME Procedure in R software. The blend had no effect (P < 0.05) on growth of R. albus and R. flavefaciens. On the other hand, S. bovis was affected by all inclusion of the blend (P < 0.05). These results provide evidence of the potential of oils to modulate the ruminal environment. Such feature can impact nutrient metabolism and, consequently, production of volatile fatty acids. Therefore, the use of a blend of clove, castor and cashew essential oils and microencapsulated active ingredients (eugenol, thymol and vanillin) used influenced the growth of gram-positive bacteria.