PSVIII-21 Modulation of Goat Plasma Proteins Using Different Preparations of Garlic

Abstract

Abstract The main objective of this study was to evaluate the effect of different garlic preparations on plasma protein concentration and Galectin secretion in goat blood. Inflammatory diseases caused by pathogens including gastrointestinal parasites impact goat gut health and productivity. Changes in plasma protein concentration are indicators of health status with application in inflammatory disease diagnosis and prognosis. Galectins (Gals) are small soluble proteins that contain one or two carbohydrate recognition domains (CRDS). ‘Prototype’ galectin-1, -2, -7, contain one CRD, Galectin 3 is chimeric, and galectin-4, -8, -9 and -12, are tandem repeat galectins with two CRDs. Galectins regulate biologic functions, immunity and disease pathogenesis and can serve as biomarkers. Garlic (Allium sativum) is a medicinal herb that has anti-parasitic and immuno-modulatory effects. Six (N=6) adult female clinically healthy Boer x Spanish goats were used. Blood was collected from the jugular vein and treated with different concentrations of fresh garlic, allicin, garlic barrier, or aged garlic (Sigma aldich). Total plasma protein secretion was evaluated using the BCA assay. Secretion of Gals 1(Prototype), Gal 3 (chimera type) and Gal 9 (tandem-repeat type) determined using commercial ELISA kits as recommended by the manufacturer. All variables were assessed using SAS analysis software (version 9.0, Sas Institute Cary, p≤0.05). Garlic treatment had a dose dependent effect on total plasma protein and Gals secretion. The protein secretion was affected by different types of garlic. Allicin (72.86μg/ml) reduced the concentration of plasma proteins. No change was observed with fresh garlic (156.334μg/ml) and garlic barrier (160.73μg/ml). Garlic barrier treatment increased protein secretion (6.3%) in goat blood. All Gals were detected (Gals 1 >9 >3), with a variable response to Garlic preparation (Garlic barrier > fresh garlic Gals secretion). Garlic modulates protein secretion. Studies are needed to determine the effect of different preparations on leukocytes and molecular targets in goat blood.