PSVII-22 Changes in biochemical composition of saliva and blood of cattle after sunflower husk included in the diet

Abstract

Abstract The aim of this study was to assess the effect of the introduction of food waste sunflower husk (SH) into the diet on the change in the chemical composition of biological media (blood and saliva) against the background of control (C). The studies were carried out on young 8-month cattle; ration of C - 70% roughage, 25% - concentrated feed, 5% - premix; SH - ration C + 10% sunflower husk (shredded, 0.5–2 mm), instead of the coarse part of the ration. The selection of biosubstrates (saliva, blood) was carried out in the morning before feeding: saliva was obtained by the method of local pharmacological stimulation of salivation (2% sodium citrate solution), blood from the jugular vein into vacuum tubes with a coagulation activator. The chemical composition of biological media was carried out on an automatic biochemical analyzer CS-T240 (Dirui Industrial Co., Ltd., China) using commercial biochemical kits for veterinary medicine (DIAKON-DS, Russia; Randox Laboratories Ltd, Great Britain). The inclusion of SH in the diet contributed to an increase in serum urea by 82.4% (P ≤ 0.05), lipase enzyme by 81.5% (P ≤ 0.05), uric acid by 62.7% (P ≤ 0.05), Ca and P by 73% and 21.8% (P ≤ 0.05), while a decrease in p-Amylase by 63.6% (P ≤ 0.05) and Fe by 86.8% (P ≤ 0.05) relative to C. After SH administration an increase in urea by 73% (P ≤ 0.05), digestive enzymes - lipase by 76.4% (P ≤ 0.05), p-amylase by 61 % (P ≤ 0.05), calcium by 69.3% and iron by 45% (P ≤ 0.05) and superoxide dismutase enzyme in comparison with C was registered in saliva. The obtained data show an increase in the level of digestive enzymes and elements in blood and saliva of ruminants, which favorably contributes to the use of sunflower husk in feeding cattle. This research was performed with financial support from the RSF (# 20-16-00088).