PSVI-8 Detoxification of zearalenone and deoxynivalenol by Biofix® Plus PRO in a molasses-based liquid feed supplement in an in vitro rumen batch culture system

Abstract

Abstract Supplementation of animal feeds with mycotoxin binders is routinely used to mitigate mycotoxin challenges. Adsorption efficiency of a binder is dependent on the structure and chemical properties of the mycotoxin, binder, and the complex gastrointestinal environment. Limited adsorption of non-polar mycotoxins has driven innovation of mitigation strategies including biotransformation or detoxification of mycotoxins by microorganisms and enzymatic activity. The current objective was to measure the efficacy of Biofix® Plus PRO (BPL) within a molasses-based liquid feed supplement to detoxify zearalenone (ZEN) and deoxynivalenol (DON) in an in vitro rumen batch culture system. Twelve reactors were filled with 1 L of liquid (50% rumen fluid, 30% reverse osmosis water, 20% synthetic saliva) and 10 g of feed (43% chopped hay, 57% corn-soy-based concentrate). Three treatments were tested in quadruplicate: Control (CON; liquid mixture only), Mycotoxin (MTX; CON + mycotoxin culture material to achieve concentration of 100 ppb ZEN and 1000 ppb DON), and BPL (MTX + 20 g/L of molasses-based liquid feed supplement including 1.65% of BPL). Samples were taken at 0 (molasses-based liquid feed added to BPL reactors after 15 min of incubation), 1, 3, 6, and 24 h and analyzed for ZEN and DON by HPLC. Results were averaged by treatment. No ZEN or DON was detected at any time point in CON group. After 3 h, ZEN was reduced by 91% and DON reduced by 87% in BPL group, with no detectable mycotoxins by 6 h. After 24 h, ZEN and DON were reduced 35% and 28%, respectively in MTX group due to natural microbial activity of the rumen fluid. Overall, the addition of Biofix® Plus PRO reduced ZEN and DON levels under the rumen batch culture conditions.