PSIV-B-39 Late-Breaking: Rhipicephalus sanguineus in domestic canines of Los Mochis, Sinaloa, Mexico

Abstract

Abstract The zoonotic potential of canine vector-borne diseases mainly tick Rhipicephalus sanguineus is associated with the strongest evidence of ticks exposed to high temperatures attach and feed on humans faster, suggesting that the risk of human parasitism by Rhipicephalus sanguineus could increase in warm weather areas, such as the state of Sinaloa, Mexico; which increases the risk of transmission of zoonotic agents as such Rocky Mountain Spotted Fever, Lyme disease, Typhus, Rickettsial, Tularemia, Babesia and Anaplasma impacting on human and canine health; so the aim of this study was genetical characterization of ticks present in dogs attended at veterinary clinics in the city of Los Mochis, Sinaloa, Mexico. for it, were collected 240 live ticks present on 120 canines and processed in the Parasitology laboratory of the FMVZ-UAS, after the Morphological identification of Rhipicephalus, were formed 5 groups and the DNA extraction was performed by QIAamp DNA Mini Kit de QIAGEN® and processed by PCR using the oligonucleotide sequences: 16S+1: 5′-CCG GTC TGA ACT CAG ATC AAG T3′ y 16S-1: 5′-GCT CAA TGA TTT TTT AAA TTG CTG T-3′, of the 16S ARNr mitochondrial. The PCR amplification of target region of the the 16S ARNr, from DNA tick samples, resulted amplicons of size 460 bp and the 5 sequences generated and analysed, was genetically compatible with Rhipicephalus sanguineus of the GenBank sequences; being important identification for the association with pathogens and the risk of transmission of canine zoonosis.