PSIV-10 Effects of Polyunsaturated Fatty Acids Supplementation in Resolvin D1 Concentration in Plasma and Follicular Fluid in Ewes

Abstract

Abstract Resolvins are lipid mediators derived from eicosapentaenoic acid and docosahexaenoic acid with anti-inflammatory effects. The hypothesis of the current experiment was that supplementation with polyunsaturated fatty acids (PUFA) would increase resolvin in plasma and follicular fluid without alterations in the macroscopic ovary observations. Thus, the aim of this study was to evaluate the concentration of resolvin D1 in plasma and follicular fluid in ewes supplemented during summer season with PUFA. In addition, examinate the macroscopic characteristic of the ovaries after PUFA supplementation. During the summer months of 2022, 24 adult ewes were blocked by body weight and body condition score and allotted in pairs into 12 pens. Within block, each pen received one of the three isocaloric treatments: supplementation with 0, 0.5, and 1% PUFA. Body weight, body condition score, and blood samples were collected every 21-days for 3 months. Blood samples were used to analyzed resolvin D1 concentration. Subsequently, ewes were synchronized and superstimulated using 240 mg of FSH administered in six decreasing doses every 12 h, and ovaries were collected by ovariectomy 12 h after the last FSH dose. Ewes were supplemented until the day of the surgery. Ovaries were weighed, measured, and follicles ≥ 5 mm and < 5 mm and corpus luteum (CL) were counted. Blood and follicular fluid samples were collected at the time of the ovariectomy. Data were analyzed as a complete randomize block design considering treatment, time, and their interaction (when possible) as fixed effects, while block and pen within block were included as random effects. Plasma and follicular fluid resolving D1 concentration were correlated using Pearson correlation (Table 1). As a results, no differences were observed in body weight (P = 0.53). There was no difference in plasma resolvin D1concentration between the different PUFA supplementation levels (P = 0.36). Conversely, there was a tendency (P = 0.06) for a lineal increase in follicular fluid resolving concentration when animals increased PUFA supplementation. In addition, there was a positive correlation between plasma and follicular fluid resolvin D1 concentration (r= 0.53; P = 0.01). There were no differences on ovarian weight (P = 0.56), ovarian volume (P = 0.80), number of follicles > 5mm (P = 0.88) or < 5mm (P = 0.32) between treatments. However, there was a quadratic effect in CL (P < 0.01) were 0% PUFA presents more structures than 0.5% and 1%. In conclusion, increasing PUFA supplementation in the diet linearly increases resolvin D1 concentration in follicular fluid, without changes in resolvin D1 concentration in plasma; however, plasma and follicular fluid concentrations of resolvin D1 are correlated.