PSIDIUM GUAJAVA L. EXTRACT INCREASES PLATELET COUNT THROUGH ENHANCEMENT OF STEM CELL FACTOR EXPRESSION IN THROMBOCYTOPENIC MICE MODEL

Nur Atik,Dany Hilmanto,Andri Reza Rahmadi,Susan Tarawifa,Erda Avriyanti

doi:10.22159/ijpps.2018v10i1.21317

Abstract

Objective: The purpose of this study was to determine the mechanism through which guava extract increases platelet count.Methods: Twenty male Swiss webster mice were divided into 4 group. Group I received 2.8 mg/20 g body weight of quinine and 1 ml of distilled water. Group II received 2.8 mg/20 grBW of quinine and 0.785 mg/20 grBW of guava extract. Group III received 1 ml distilled water and 0.785 mg/20 grBW of guava extract and Group IV only received 1 ml distilled water. After 14 days** treatment, platelet count was measured using Raas-Ecker method. SCF and TPO mRNA expressions in mice liver were assessed using qRT-PCR.Results: The data showed that quinine administration reduced platelet count significantly (P<0.05). Psidium guajava L. extraction significantly enhanced platelet count in mice that were treated with quinine compared with quinine group (p<0.05). The mechanism was analysed further and our RT PCR data showed that stem cell factor mRNA expression significant increased (p<0.05) in quinine plus guava extract group compared to quinine group.Conclusion: It indicated guava extract role in increasing megakaryopoiesis which resulted in an increase of platelet count.

Highlights

Platelets are small anucleate cell fragments that are discoid in shape, 1 to 3 μm in diameter
After evaluated we found that fruits have taxonomy as Psidium guajava L. with Myrtaceae as family and Myrtalesas order
To evaluate the effect of guava extract in increasing platelet count, we compared the counts from mice after treatment with quinine and guava extracts

Summary

Introduction

Platelets are small anucleate cell fragments that are discoid in shape, 1 to 3 μm in diameter. Formed from the cytoplasm of megakaryocytes (MKs) which reside in the bone marrow, they are indispensable for processes such as haemostasis, wound healing, angiogenesis, inflammation, and innate immunity [1]. The average platelet number in humans ranges from 150 × 109 to 400 × 109 per liter [2]. Platelet generation is a two-stage process involving the differentiation of hematopoietic stem cells into mature megakaryocytes and release of platelets from MKs [3]. Stem cell factor (SCF) and thrombopoietin (TPO) are critical cytokines in regulating stem cell proliferation and survival of progenitor cell during hematopoiesis. TPO is an important megakaryocytic cytokine affecting the megakaryocytic differentiation [4]

Methods

Results

Conclusion