Abstract
P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1+ cells
Highlights
The ongoing coronavirus disease 2019 (COVID-19) is a global pandemic afflicting more than 63 million people in over 200 countries and territories, resulting in more than1.47 million deaths as of 1 Decmber 2020
Previous studies have demonstrated that P-selectin glycoprotein ligand-1 (PSGL-1) can be incorporated into HIV-1 virions, and its virion incorporation subsequently blocks particle binding to target cells and infectivity [1,5]
The infectivity of PSGL-1-imprinted SARS-CoV particles was completely abrogated in Vero cells (Figure 1D), demonstrating the ability of PSGL-1 to block the infectivity of SARS-CoV S-bearing HIV-1 particles
Summary
The ongoing coronavirus disease 2019 (COVID-19) is a global pandemic afflicting more than 63 million people in over 200 countries and territories, resulting in more than1.47 million deaths as of 1 Decmber 2020. The ongoing coronavirus disease 2019 (COVID-19) is a global pandemic afflicting more than 63 million people in over 200 countries and territories, resulting in more than. Efforts to develop effective treatments and vaccines are moving forward rapidly, SARS-CoV-2, the virus that causes COVID-19, continues to spread. Understanding virus–host interactions is critical to developing both therapeutics and vaccines aimed at containing the pandemic. P-selectin glycoprotein ligand-1 (PSGL-1, known as SELPLG or CD162) is a host protein recently identified to possess broad-spectrum antiviral activity [1]. PSGL-1 binds to the selectin family of proteins, P-, E-, and L-selectin [2], and mediates immune cell tethering and rolling on endothelium’s surface promote cell migration into inflamed tissues [3]. In the context of viral infection, PSGL-1 has been identified as an IFN-γ-regulated inhibitory factor involved in blocking the infectivity of HIV-1 [4], and other viruses [1], through the steric hindrance of particle attachment to target cells [1,5]
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