Abstract

A cellulase free, alkaline, thermo-tolerant pectinase was produced by a novel yeast strain Pseudozyma sp. SPJ using citrus peel as inexpensive carbon source. The crude enzyme showed good prospects in degumming of flax fibers for textile industry. An optimum pectinase dose of 80 U g−1 resulted in reduction of 15 ± 1.92% dry weight of the fibers, releasing maximum galacturonic acid (10825.5 ± 34.2 μg g−1 dry fiber) after the incubation of 6 h. The yeast culture could grow on the flax fibers (as sole carbon source) without addition of any other nutrient and produce good enzyme yield (9235.5 ± 21.51 U g−1 dry fiber). After 12 h incubation of the fibers with the isolated yeast strain, 4471 ± 19.5 μg g−1 dry fiber galacturonic acid was achieved with maximum weight loss of 11 ± 1.2%. This process reduced the amount of chemicals and energy used in conventional methods. It also contributed to enhance fineness and overall quality of the fiber strands. This study is relevant to the textile industry as it provided a fast, economical and eco-friendly method for degumming of flax fibers.

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