Pseudovibrio axinellae sp. nov., isolated from an Irish marine sponge

John A O’Halloran,John P Morrissey,Fergal O’Gara,Teresa M Barbosa,Jonathan Kennedy,Alan D W Dobson

doi:10.1099/ijs.0.040196-0

John A O’Halloran, John P Morrissey + Show 4 more

Open Access

https://doi.org/10.1099/ijs.0.040196-0

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Abstract

A Gram-negative, motile, rod-shaped bacterial strain, designated Ad2(T), was isolated from a marine sponge, Axinella dissimilis, which was collected from a semi-enclosed marine lake in Ireland. Strain Ad2(T) grew optimally at 24 °C, at pH 7.0 and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Ad2(T) clustered with members of the genus Pseudovibrio, and showed 97.3-98.2 % sequence similarity to the type strains of recognized Pseudovibrio species. DNA-DNA relatedness values between strain Ad2(T) and the type strains of other Pseudovibrio species were <27 %. The DNA G+C content of strain Ad2(T) was 50.5 mol%. The major fatty acid was 18 : 1ω7c. Differences in phenotypic properties, together with phylogenetic and DNA-DNA hybridization analyses, indicated that strain Ad2(T) represented a novel species of the genus Pseudovibrio. The name Pseudovibrio axinellae sp. nov. is proposed, with Ad2(T) (= DSM 24994(T) = NCIMB 14761(T)) as the type strain.

Highlights

Based on 16S rRNA gene sequencing and randomly amplified polymorphic DNA analysis
Transmission electron microscopy of cells of strain Ad2T negatively stained with uranyl acetate showed that, to the previously reported type strains of species of the genus Pseudovibrio, strain Ad2T cells possessed one to several subpolar flagella (Fig. S1, available in IJSEM Online)
The neighbour-joining phylogenetic analysis indicated that strain Ad2T was a member of the genus Pseudovibrio (Fig. 1), and this was supported by the maximumparsimony phylogenetic analysis, where a similar tree topology was observed (Fig. S2)

Summary

Introduction

Based on 16S rRNA gene sequencing and randomly amplified polymorphic DNA analysis. Here, we report on the characterization of strain Ad2T using a polyphasic approach. Colonies of strain Ad2T were circular, smooth and beige in colour with an entire margin on SYP-SW agar. The levels of 16S rRNA gene sequence similarity between strain Ad2T and P. ascidiaceicola F423T, P. denitrificans DN34T and P. japonicus WSF2T were calculated using the EzTaxon-e tool (Kim et al, 2012) as 98.2, 97.3 and 98.3 %, respectively.

Results

Conclusion