Abstract
In continuation of our search for new bioactive compounds from soil microbes, a fluorescent Pseudomonas strain isolated from paddy field soil of Kuttanad, Kerala, India was screened for the production of bioactive secondary metabolites. This strain was identified as Pseudomonas mosselii through 16S rDNA gene sequencing followed by BLAST analysis and the bioactive metabolites produced were purified by column chromatography (silica gel) and a pure bioactive secondary metabolite was isolated. This bioactive compound was identified as Pseudopyronine B by NMR and HR-ESI-MS. Pseudopyronine B recorded significant antimicrobial activity especially against Gram-positive bacteria and agriculturally important fungi. MTT assay was used for finding cell proliferation inhibition, and Pseudopyronine B recorded significant antitumor activity against non-small cell lung cancer cell (A549), and mouse melanoma cell (B16F10). The preliminary MTT assay results revealed that Pseudopyronine B recorded both dose- and time-dependent inhibition of the growth of test cancer cell lines. Pseudopyronine B induced apoptotic cell death in cancer cells as evidenced by Acridine orange/ethidium bromide and Hoechst staining, and this was further confirmed by flow cytometry analysis using Annexin V. Cell cycle analysis also supports apoptosis by inducing G2/M accumulation in both A549 and B16F10 cells. Pseudopyronine B treated cells recorded significant up-regulation of caspase 3 activity. Moreover, this compound recorded immunomodulatory activity by enhancing the proliferation of lymphocytes. The production of Pseudopyronine B by P. mosselii and its anticancer activity in A549 and B16F10 cell lines is reported here for the first time. The present study has a substantial influence on the information of Pseudopyronine B from P. mosselii as potential sources of novel drug molecule for the pharmaceutical companies, especially as potent antimicrobial and anticancer agent.
Highlights
T Research, University of Sharjah, Sharjah, UAE E Specialty section: This article was submitted to R Antimicrobials, Resistance
In continuation of our search for new bioactive compounds from soil microbes, a fluorescent Pseudomonas strain isolated from paddy field soil of Kuttanad, Kerala, India was screened for the production of bioactive secondary metabolites
This strain was identified as Pseudomonas mosselii through 16S rDNA gene sequencing followed by BLAST analysis and the bioactive metabolites produced were purified by column chromatography and a pure bioactive secondary metabolite was isolated
Summary
Agriculture.” the search for new antimicrobials from natural sources is an important area of many researchers. Microorganisms from soils play an important source severity of a broad range of infectious diseases caused by of many novel antibiotic compounds due to their high abundance human pathogenic microbes. There are many reports of the production spread use of antibiotics, the competence of many introduced of antimicrobial metabolites by Pseudomonas spp. To and Murray, 2009; Fischbach and Walsh, 2009) Infectious achieve this goal, several new sources of antimicrobial/anticancer diseases account for more than 13 billion human deaths compounds ranging from natural to synthetic are currently worldwide, which accounts for about 25% of all deaths The antibiotic treatments of various infectious for new bioactive secondary metabolites from soil bacteria, diseases are getting limited due to the reemergence of Pseudopyronine B was purified from a Pseudomonas mosselii TR multi-drug-resistance (MDR) pathogenic microbes, which strain. R death among humans (males and females) and the single major and anticancer activity of Pseudopyronine B
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