Abstract

Some types of nanoparticles such as gold and silver show interesting chemical properties. Pseudoperoxidase activity has been found in iron- or gold-based materials, but some silver nanoparticles (AgNPs) also exhibit such properties. Pseudoperoxidase activity of AgNPs prepared by green synthesis using sage leaves was studied. The AgNPs (prepared by green synthesis using sage leaves) size ranged from 20 to 50 nm and zeta potential was found to vary from -10 to 20 mV, with absorption maximum being 430 nm. The peroxidase-like activity was monitored in the presence of 5 mM 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (30%). The nanoparticles showed about 80% activity of 1 mU horseradish peroxidase (HRP). In addition, AgNPs were applied to maize seedlings (5 days in hydroponics system) at concentrations of 1 mg/L, 50 mg/L, and 150 mg/L. Peroxidase activity in leaves after 48 hours was 59.5, 67.5, and 57.2 µg.mL-1 of HRP, respectively. Peroxidase activity of untreated plant leaves was 58.8 µg.mL-1 of HRP. Peroxidase activity in roots after 48 hours was 101.6, 109.0, and 156.7 µg.mL-1 of HRP, respectively. The peroxidase activity level of the control variant in root of treatment was 98.7 µg.mL-1 of HRP. The results indicate that the determination of peroxidase activity could be used to assess the phytotoxicity of nanoparticles in plants.

Highlights

  • With the development of nanotechnologies and their subsequent applications in biology, there is considerable interest in their use in nanomedicine, in particular in the field of drug delivery, imaging or biological separation techniques

  • Pseudoperoxidase activity has been found in iron- or gold-based materials, but some silver nanoparticles (AgNPs) exhibit such properties

  • Pseudoperoxidase activity of AgNPs prepared by green synthesis using sage leaves was studied

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Summary

Introduction

With the development of nanotechnologies and their subsequent applications in biology, there is considerable interest in their use in nanomedicine, in particular in the field of drug delivery, imaging or biological separation techniques. Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials in various fields. Silver nanoparticles exhibit significant antibacterial effects thereby providing excellent potential for industrial applications [2]. HRP is the most widely used enzyme in biotechnological applications where it provides a color reaction in the presence of hydrogen peroxide and a suitable chromogen 3,3',5,5'-tetramethylbenzidine (TMB) [12]. The peroxidase-like activity has been reported in some types of nanoparticles, such as iron or gold [13,14]. The advantage of such particles is their excellent stability at normal and extreme temperatures, pH, etc. The aim of this work was to study the effect of different concentrations of AgNPs and silver nitrate (AgNO3) on maize seedlings (Zea mays) and the assessment of their peroxidase activity

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