Abstract
A Gram-positive, aerobic, actinobacterial strain with rod-shaped spores, CAP47RT, which was isolated from the surface-sterilized root of a native pine tree (Callitris preissii), grown inSouth Australia is described. The major cellular fatty acid of this strain was iso-H-C16:1 and major menaquinone was MK-8(H4). The diagnostic diamino acid in thecell-wallpeptidoglycan wasidentifiedasmeso-diaminopimelicacid. These chemotaxonomic data confirmed the affiliation of strain CAP47RT to the genus Pseudonocardia. Phylogenetic evaluation based on 16S rRNA gene sequence analysis placed this strain in the family Pseudonocardiaceae, being most closely related to Pseudonocardia xishanensis JCM 17906T (98.8%), Pseudonocardia oroxyli DSM 44984T (98.7%), Pseudonocardia thailandensis CMU-NKS-70T (98.7%), and Pseudonocardia ailaonensis DSM 44979T (97.9%). The results of the polyphasic study which contain genome comparisons of ANIb, ANIm, and digital DNA-DNA hybridization revealed the differentiation of strain CAP47RT from the closest species with validated names. This strain represents a novel species and the name proposed for this microorganism is Pseudonocardia pini sp. nov., indicating the source ofthis actinobacterium from a pine tree. The type strain is CAP47RT (= DSM 108967T = NRRL B-65534T). Genome mining revealed that this strain contained a variety of genes encoding enzymes that candegrade hazardous chemicals.
Highlights
The genus Pseudonocardia belonging to family Pseudonocardiaceae was first proposed by Henssen (1957)
The endophytic actinobacterium, strain CAP47RT was discovered in the course of a study which focused on the biodiversity of endophytic actinobacteria from Australian native trees and screening for antimicrobial activity (Kaewkla and Franco 2013)
Reference strains; P. oroxyli DSM 44984T and P. ailaonensis DSM 44979T were obtained from German Collection of Microorganisms and Cell Cultures GmbH (DSMZ) and Pseudonocardia xishanensis JCM 17906T was obtained from Japan Collection of Microorganisms RIKEN BioResource Research Center (JCM)
Summary
The genus Pseudonocardia belonging to family Pseudonocardiaceae was first proposed by Henssen (1957). It is the only family in the Order Pseudonocardiales (Labeda and Goodfellow 2012) 2014). At the time of submission of this manuscript, the genus encompasses more than 60 valid species (https://lpsn.dsmz.de/genus/Psedonocardia) (Parte et al 2020). They were discovered from different environmental samples including soil, marine sediments, activated sludge, termite nets and gold mine core including plant tissues. The description of the phenotypic and genotypic properties including a genome comparative study of a Pseudonocardia-like strain, CAP47RT, is presented. The data show that this strain represents a novel species, for which the name Pseudonocardia pini sp. nov. is proposed
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